![]() The inoculated leaves initially developed symptoms of chlorosis that progressed to necrosis. The DNA of pure cultures was extracted and submitted to PCR using the primers ITS1 and ITS4. Symptom development was assessed on a daily basis, followed by reisolation. ![]() In pathogenicity testing, healthy leaves were inoculated with mycelial disks and controls with disks containing potato dextrose agar (PDA) medium. Three morphologically similar fungal isolates were obtained. obesum leaves with necrotic spots on the edges and/or tips were collected for indirect isolation of the pathogen. The aim of this study was to report the first occurrence of Pestalotiopsis microspora as the causal agent of leaf spots on desert rose. However, diseases can be a limiting factor because they affect crop quality and yield. Among the main ornamental plants grown in Brazil, Adenium obesum stands out for its appearance, drought resistance and beautiful flowers.
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